Color TSFG microscopy of red blood cells and oxygenation

Júlia Ferrer Ortas; Pierre Mahou; Sophie Escot; Chiara Stringari; Nicolas B. David; Laure Bally-Cuif; Nicolas Dray; Michel Négrerie; Willy Supatto; Emmanuel Beaurepaire

doi:10.1117/12.2670229

1 September 2023 Color TSFG microscopy of red blood cells and oxygenation

Júlia Ferrer Ortas, Pierre Mahou, Sophie Escot, Chiara Stringari, Nicolas B. David, Laure Bally-Cuif, Nicolas Dray, Michel Négrerie, Willy Supatto, Emmanuel Beaurepaire

Author Affiliations +

Proceedings Volume 12630, Advances in Microscopic Imaging IV; 1263002 (2023) https://doi.org/10.1117/12.2670229
Event: European Conferences on Biomedical Optics, 2023, Munich, Germany

Abstract

We present color third-order sum-frequency generation (color TSFG) microscopy, a multiphoton imaging strategy based on the simultaneous detection of several third-order coherent signals produced by two synchronized femtosecond pulse trains. We demonstrate that it can be used to obtain red blood cell (RBC)-specific label-free contrast in live zebrafish and is a promising tool for probing RBC oxygenation.

Citation Download Citation

Júlia Ferrer Ortas, Pierre Mahou, Sophie Escot, Chiara Stringari, Nicolas B. David, Laure Bally-Cuif, Nicolas Dray, Michel Négrerie, Willy Supatto, and Emmanuel Beaurepaire "Color TSFG microscopy of red blood cells and oxygenation", Proc. SPIE 12630, Advances in Microscopic Imaging IV, 1263002 (1 September 2023); https://doi.org/10.1117/12.2670229

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