Image recovery of fluorescent beads by TIE-based computational imaging and phase retrieval

Shiori Matsuda; Naru Yoneda; Manoj Kumar; Xiangyu Quan; Wataru Watanabe; Osamu Matoba

doi:10.1117/12.3007915

20 September 2023 Image recovery of fluorescent beads by TIE-based computational imaging and phase retrieval

Shiori Matsuda, Naru Yoneda, Manoj Kumar, Xiangyu Quan, Wataru Watanabe, Osamu Matoba

Proceedings Volume 12608, Biomedical Imaging and Sensing Conference; 126080V (2023) https://doi.org/10.1117/12.3007915
Event: Optics and Photonics International Congress, 2023, Yokohama, Japan

Abstract

A degraded fluorescence image passing through a weak diffuser is improved by the acquisition of fluorescence wavefront information and phase retrieval. The amplitude and phase distributions of the fluorescent light wave are obtained using the transport of intensity equation. After calculating back-propagation to the diffuser surface from the recording plane, phase retrieval is applied for improving the image quality. Experiments using fluorescent beads examined at different recording plane showed the effectiveness of the proposed method.

Citation Download Citation

Shiori Matsuda, Naru Yoneda, Manoj Kumar, Xiangyu Quan, Wataru Watanabe, and Osamu Matoba "Image recovery of fluorescent beads by TIE-based computational imaging and phase retrieval", Proc. SPIE 12608, Biomedical Imaging and Sensing Conference, 126080V (20 September 2023); https://doi.org/10.1117/12.3007915

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