Imaging radiobiological response of breast cancer cells in vitro using stimulated Raman scattering

C. Harry Allen; Robyn Skillings; Duale Ahmed; Sarita C. Sanchez; Kaitlyn Altwasser; Justin Gagnon; Vinita Chauha; Edana Cassol; Sangeeta Murugkar

doi:10.1117/12.2650138

15 March 2023 Imaging radiobiological response of breast cancer cells in vitro using stimulated Raman scattering

C. Harry Allen, Robyn Skillings, Duale Ahmed, Sarita C. Sanchez, Kaitlyn Altwasser, Justin Gagnon, Vinita Chauha, Edana Cassol, Sangeeta Murugkar

Author Affiliations +

Proceedings Volume 12392, Advanced Chemical Microscopy for Life Science and Translational Medicine 2023; 1239204 (2023) https://doi.org/10.1117/12.2650138
Event: SPIE BiOS, 2023, San Francisco, California, United States

Abstract

Radiation therapy (RT) is a standard treatment for ~50% of cancers worldwide. The major goal in RT is to maximize tumor cell kill while minimizing radiation-induced damage to healthy tissue. However, several fundamental questions remain unanswered related to the radiation-matter interactions, and the ensuing radiobiological responses in cells and tissue. Stimulated Raman scattering (SRS) microscopy is a promising technology for imaging the heterogeneous metabolic responses in cells exposed to ionizing radiation. In this work, we demonstrate SRS imaging of endogenous macromolecules such as lipids and proteins in the C-H stretching region (2800 to 3100 cm^-1) in MCF-7 breast cancer cells in vitro. We compare the response of control (unirradiated) cells and cells irradiated at 30 Gy (120 kVp x-rays), at three time points of 24, 48, and 72 hours post exposure to ionizing radiation, and find significant changes between irradiated and control MCF-7 cells over time.

Conference Presentation

Citation Download Citation

C. Harry Allen, Robyn Skillings, Duale Ahmed, Sarita C. Sanchez, Kaitlyn Altwasser, Justin Gagnon, Vinita Chauha, Edana Cassol, and Sangeeta Murugkar "Imaging radiobiological response of breast cancer cells in vitro using stimulated Raman scattering", Proc. SPIE 12392, Advanced Chemical Microscopy for Life Science and Translational Medicine 2023, 1239204 (15 March 2023); https://doi.org/10.1117/12.2650138

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