High-resolution label-free cell imaging with angular information encoded dynamic speckle illumination

Xiantao Jiang; Nansen Zhou; Yijin Wang; Liting Duan; Renjie Zhou

doi:10.1117/12.2648109

16 March 2023 High-resolution label-free cell imaging with angular information encoded dynamic speckle illumination

Xiantao Jiang, Nansen Zhou, Yijin Wang, Liting Duan, Renjie Zhou

Proceedings Volume 12389, Quantitative Phase Imaging IX; 123890H (2023) https://doi.org/10.1117/12.2648109
Event: SPIE BiOS, 2023, San Francisco, California, United States

Conference Poster

Abstract

Laser-based wide-field coherent imaging methods suffer from low image contrast due to the speckle noise as well as poor lateral resolution using collimated illumination. To improve the image contrast and spatial resolution for label-free cell imaging applications, we propose a new dynamic speckle illumination scheme using perfect optical vortex (POV) beams that can provide finer speckles with more uniform distributions. The low spatial coherence from the POV speckle field has significantly improved the signal-to-noise ratio (SNR) and the image contrast, thus contributing to a high spatial resolution that matches the diffraction limit in our cell imaging experiments. Importantly, the depth-resolved imaging capability has been realized which has allowed us to visualize fine subcellular structures at different focal planes.

Citation Download Citation

Xiantao Jiang, Nansen Zhou, Yijin Wang, Liting Duan, and Renjie Zhou "High-resolution label-free cell imaging with angular information encoded dynamic speckle illumination", Proc. SPIE 12389, Quantitative Phase Imaging IX, 123890H (16 March 2023); https://doi.org/10.1117/12.2648109

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available