Optical clearing agent enhanced multimodal photoacoustic microscopy and optical coherence tomography imaging of rabbit eyes

Wei Qin; Fengxian Du; Yuchen Wang; Xueqing Ding; Tianyang Zhao; Cuixia Dai

doi:10.1117/12.2642236

19 December 2022 Optical clearing agent enhanced multimodal photoacoustic microscopy and optical coherence tomography imaging of rabbit eyes

Wei Qin, Fengxian Du, Yuchen Wang, Xueqing Ding, Tianyang Zhao, Cuixia Dai

Author Affiliations +

Proceedings Volume 12320, Optics in Health Care and Biomedical Optics XII; 123201S (2022) https://doi.org/10.1117/12.2642236
Event: SPIE/COS Photonics Asia, 2022, Online Only

Conference Poster

Abstract

Multimodal imaging photoacoustic microscopy (PAM) and optical coherence tomography (OCT) can provide morphological and functional information of living organisms which reveals the physiological and pathological state of biological tissues. In this study, a dual-modal PAM and OCT system was custom-built to simultaneously image the optical absorption and scattering characteristic of rabbit eyes. Glycerol solution was used as optical clearing agent to enhance PAM and OCT imaging of New Zealand White rabbit eyes in vivo. Results showed that both OCT and PAM signals were increased by retro-bulbar injection of glycerol to New Zealand White rabbit eyes. The PAM signal amplitude increased from 0.1476 (a.u) to 0.1701 (a.u) after glycerol solution injection, which increased by 115%. And intensity of OCT signal was increased up to 150%. The optical clearing agent enhanced multimodal imaging technique provides a new method to increase the depth of optical measured depth and to improve the imaging contrast, which can be used to evaluate pathophysiological early symptoms in vivo.

Citation Download Citation

Wei Qin, Fengxian Du, Yuchen Wang, Xueqing Ding, Tianyang Zhao, and Cuixia Dai "Optical clearing agent enhanced multimodal photoacoustic microscopy and optical coherence tomography imaging of rabbit eyes", Proc. SPIE 12320, Optics in Health Care and Biomedical Optics XII, 123201S (19 December 2022); https://doi.org/10.1117/12.2642236

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