A high sensitivity method for detecting IL-4 and IL-6 in serum of uremic patients

Shuang-song Lv; Shih-Mo Yang

doi:10.1117/12.2636648

10 June 2022 A high sensitivity method for detecting IL-4 and IL-6 in serum of uremic patients

Shuang-song Lv, Shih-Mo Yang

Proceedings Volume 12179, Second International Conference on Medical Imaging and Additive Manufacturing (ICMIAM 2022); 1217906 (2022) https://doi.org/10.1117/12.2636648
Event: Second International Conference on Medical Imaging and Additive Manufacturing (ICMIAM 2022), 2022, Xiamen, China

Abstract

Uremia is a serious disease in the stage of renal failure, so it is very important to detect uremia in time. In order to overcome the shortcomings of insufficient sensitivity of traditional ELISA methods, we report a digital ELISA detection platform composed of three parts of microsphere operating system, optical imaging system and computer processing system, which can realize the automatic detection of biomarkers in serum. The platform can combine fluorescent coding microspheres with microporous array, and apply DEP electric field externally, which can effectively increase the recognition efficiency of fluorescent coding microspheres and realize the ultra-high sensitivity detection of two important cytokines of IL-6 and IL-4 in uremic serum. The experiment successfully and accurately detected the IL-4 and IL-6 in the serum of uremic patients. At the end of the article, we summarize and prospect the experiment.

Citation Download Citation

Shuang-song Lv and Shih-Mo Yang "A high sensitivity method for detecting IL-4 and IL-6 in serum of uremic patients", Proc. SPIE 12179, Second International Conference on Medical Imaging and Additive Manufacturing (ICMIAM 2022), 1217906 (10 June 2022); https://doi.org/10.1117/12.2636648

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