Opto-chemical disinfection of bacterial pathogens in mature biofilms grown in flexible endoscope

Van Nam Tran; Periaswamy Sivagnanam Saravana; Suhyun Park; Fazlurrahman Khan; Van Gia Truong; Seok Jeong; Don Haeng Lee; Byung-Soo Chun; Young Mog Kim; Hyun Wook Kang

doi:10.1117/12.2626049

11 January 2022 Opto-chemical disinfection of bacterial pathogens in mature biofilms grown in flexible endoscope

Van Nam Tran, Periaswamy Sivagnanam Saravana, Suhyun Park, Fazlurrahman Khan, Van Gia Truong, Seok Jeong, Don Haeng Lee, Byung-Soo Chun, Young Mog Kim, Hyun Wook Kang

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Proceedings Volume 12159, SPIE Advanced Biophotonics Conference (SPIE ABC 2021); 121590C (2022) https://doi.org/10.1117/12.2626049
Event: SPIE Advanced Biophotonics Conference (SPIE ABC 2021), 2021, Busan, Republic of Korea

Abstract

Flexible endoscope reprocessing is an important requirement to minimize the risk of cross-infection between patients due to incomplete disinfection of a bacteria biofilm. The present study introduces a novel opto-chemical treatment to disinfect microbial biofilms (both Gram-positive and Gram-negative bacterial biofilms), commonly found in flexible endoscopes. A low concentration disinfectant combined with infrared and blue light irradiations was applied to disinfect the bacterial biofilms in the endoscope. A basket-integrated optical device was designed to deliver uniform and concentric light onto the channel surface of the endoscope. Colony-forming unit assays were performed to quantify the vial cells while scanning electron microscopy (SEM) illustrated an extracellular matrix (ECM) of the bacterial biofilm. The infrared light irradiation heated the surface of the bacterial biofilm to ~ 65°C. The blue light irradiation induced a relative temperature increase of 30°C on the bacterial biofilm. The results showed that the opto-chemical treatment reduced approximately 7.5-log₁₀ of the bacterial biofilm, which was four times higher than that of a standard disinfectant solution (2% glutaraldehyde). In comparison with the control untreated samples with intact ECMs, the SEM images showed significant damage to the bacterial biofilm under the opto-chemical treatment. The combined treatment induced antimicrobial effects in terms of inhibition of protein synthesis, thermal destruction, and oxidative stress, eradicating the bacterial biofilm more than the standard chemical disinfection. The proposed technique could be an alternative approach to disinfect the microbial biofilms and minimize the risk of secondary infection in endoscopy-related medical facilities.

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Van Nam Tran, Periaswamy Sivagnanam Saravana, Suhyun Park, Fazlurrahman Khan, Van Gia Truong, Seok Jeong, Don Haeng Lee, Byung-Soo Chun, Young Mog Kim, and Hyun Wook Kang "Opto-chemical disinfection of bacterial pathogens in mature biofilms grown in flexible endoscope", Proc. SPIE 12159, SPIE Advanced Biophotonics Conference (SPIE ABC 2021), 121590C (11 January 2022); https://doi.org/10.1117/12.2626049

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KEYWORDS

Endoscopes

Scanning electron microscopy

Bacteria

Infrared radiation

Pathogens

Temperature metrology

Biomimetics

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