An integrated top-stage incubator and lens-free holographic imaging system for culture monitoring applications

Zeynep Ozbilgin; Sevdenur Aksit; M. Fatih Toy

doi:10.1117/12.2622639

20 May 2022 An integrated top-stage incubator and lens-free holographic imaging system for culture monitoring applications

Zeynep Ozbilgin, Sevdenur Aksit, M. Fatih Toy

Author Affiliations +

Proceedings Volume 12136, Unconventional Optical Imaging III; 121360V (2022) https://doi.org/10.1117/12.2622639
Event: SPIE Photonics Europe, 2022, Strasbourg, France

Abstract

Lens free inline holographic microscopy was shown to be a potent approach for many applications relying on cellular imaging. Applications requiring a large field of view at moderate resolution are the ones that are most suitable for this platform. Besides, the simplicity of the overall imaging system, which requires only a light source and a camera, positions this approach as an easily accessible one. Acquired holograms from such a system are processed to recover phase images. As an additional advantage on top of the simplicity, phase imaging enables the imaging of otherwise transparent cell samples without any need for labeling or staining. Eventually, such a system can be used for long term imaging of live cell cultures with wide field of view. Up to now, two alternatives were explored for the imaging of live cell cultures for extended duration. In one approach, a portable imaging system was placed inside a standard incubator with cell cultures on top. A stage top incubator was used on a modified microscope in the other approach. In both approaches, the cost of the system grows due to commercial systems, and the overall footprint of the system with incubator is too large to be classified as portable. Here, an integrated portable system is presented that can maintain cell cultures at desired temperature in a 3D printed enclosure while imaging them in lens free inline holographic microscopy modality. Such a system is well suited for tissue culturing and monitoring at limited resources settings.

Conference Presentation

Citation Download Citation

Zeynep Ozbilgin, Sevdenur Aksit, and M. Fatih Toy "An integrated top-stage incubator and lens-free holographic imaging system for culture monitoring applications", Proc. SPIE 12136, Unconventional Optical Imaging III, 121360V (20 May 2022); https://doi.org/10.1117/12.2622639

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