Optical methods for assessing the effect of DON-1R on the histological structure of fish liver

L. Khuda; O. Khudyi; L. Cheban

doi:10.1117/12.2615589

20 December 2021 Optical methods for assessing the effect of DON-1R on the histological structure of fish liver

L. Khuda, O. Khudyi, L. Cheban

Proceedings Volume 12126, Fifteenth International Conference on Correlation Optics; 1212618 (2021) https://doi.org/10.1117/12.2615589
Event: Fifteenth International Conference on Correlation Optics, 2021, Chernivtsi, Ukraine

Abstract

With the help of optical methods of fish functional state diagnostics the possibility of using DON-1R, a drug that contains γ–crotonolactone, in recirculating aquaculture system was proven. It was stated that adding DON-1R to granulated feed while feeding sturgeon fish stimulates the raise in mass accumulating velocity. Moreover, microscopical analysis of histological cuts and detailed analysis of their photomicrographs with the use of various approaches in contrasting images showed that feeding with feed with DON-1R is accompanied with reactive changes to the liver. In particular, fish that received the tested drug had the inequable liver cytoarchitectonics, chaotic hepatocytes placement, radial character of liver channels and undefined sinusoids. Hepatocytes of experimental fish had large hyperchromatic nuclei with 4,5 μm diameter with multiple tiny defined nucleoli. Control fish’s nuclei were 1,5 times smaller, had even coloring and 1-2 nucleoli. Moreover, fish from experimental group had signs of partial acidophilic coagulation, such as occurrence of homogenous pink lumps in cytoplasm (Mallory bodies). It is worth noting that the application of DON-1R does not lead to deep destructive changes in liver structure, such as necrosis of fibrosis. Taking all of the above into consideration, DON-1R is the most effective for growth stimulation if applied for a short period of time for commercial growing. Optimal period of use is 20 days.

Citation Download Citation

L. Khuda, O. Khudyi, and L. Cheban "Optical methods for assessing the effect of DON-1R on the histological structure of fish liver", Proc. SPIE 12126, Fifteenth International Conference on Correlation Optics, 1212618 (20 December 2021); https://doi.org/10.1117/12.2615589

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
7 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Liver

Tissues

Blood

Diagnostics

Photomicroscopy

Spectrophotometry

Microscopy

Show All Keywords

Keywords/Phrases

Search In:

Publication Years