Detection of microparticles in flow by 2D light scattering and fluorescence imaging

Xin Qi; Zhuo Wang; Xuantao Su

doi:10.1117/12.2606539

1 November 2021 Detection of microparticles in flow by 2D light scattering and fluorescence imaging

Xin Qi, Zhuo Wang, Xuantao Su

Proceedings Volume 12057, Twelfth International Conference on Information Optics and Photonics; 120573J (2021) https://doi.org/10.1117/12.2606539
Event: Twelfth International Conference on Information Optics and Photonics, 2021, Xi'an, China

Abstract

The in-flow detection of microparticles is of great importance in biomedical fields. Previous studies have shown that twodimensional (2D) light scattering technology has the capability to detect and differentiate unlabeled cells, while fluorescence imaging can be used as a specific cytological detection method. Here, we built a system to acquire simultaneously the 2D light scattering patterns and fluorescence images of single microparticles in flow. A 488 nm laser beam was reshaped into a light sheet by a cylindrical lens, which was used to excite the microparticles in a microfluidic tube. The 2D light scattering and fluorescence signals were collected by a 10x objective lens through a 505 nm dichroic beam splitter, and they were simultaneously acquired by dual complementary metal oxide semiconductor (CMOS) detectors. Our system acquired the 2D light scattering patterns and fluorescence images of single microparticles from a mixture (2 μm fluorescent particles, 2 μm and 3.87 μm non-fluorescent particles) in flow. Based on the 2D light scattering patterns and the fluorescence images, the number and the ratio of mixed microparticles can be determined. Moreover, the label-free microparticle size differentiation can also be achieved with the 2D light scattering patterns. The detection of microparticles in flow that combines label-free 2D light scattering technology and fluorescence imaging may find potential applications for single cell analysis.

Citation Download Citation

Xin Qi, Zhuo Wang, and Xuantao Su "Detection of microparticles in flow by 2D light scattering and fluorescence imaging", Proc. SPIE 12057, Twelfth International Conference on Information Optics and Photonics, 120573J (1 November 2021); https://doi.org/10.1117/12.2606539

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