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4 March 2022 Comparative study of proteinaceous microfabrication with different repetition rates by femtosecond direct laser write
Daniela Serien, Aiko Narazaki
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Proceedings Volume 11992, Laser 3D Manufacturing IX; 1199208 (2022) https://doi.org/10.1117/12.2610445
Event: SPIE LASE, 2022, San Francisco, California, United States
Abstract
Femtosecond laser direct write (fs-LDW) is a promising technique of 3D printing of biomaterials such as protein due to nonlinear multiphoton absorption processes facilitating microfabrication along a designated laser light path. To use protein as precursor material for fs-LDW is attractive because the fabricated structures retain their native function as demonstrated by several reports. These reports range over a select variety of protein and photoactivators, but pure protein can also be utilized as precursor. The resulting proteinaceous microstructures with retained native function and submicron feature sizes might offer diverse biomedical or biochip applications. Based on the review of previous publications, it seems that both MHz and kHz range have been used to fabricate microstructures. In our body of work, we considered so far fluence and total accumulated fluence to be the key parameter to control fabrication success and fine-tune feature sizes. Here, we aim to explore whether repetition rate is a significantly contributing parameter to the process of proteinaceous microfabrication by fs-LDW. We fabricate microstructure arrays from the protein bovine serum albumin for repetition rates between 10kHz and 1MHz with constant pulse width and pulse energies from 20 to 100 nJ. We find that 100kHz is the optimal repetition rate for our protein precursor.
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Daniela Serien and Aiko Narazaki "Comparative study of proteinaceous microfabrication with different repetition rates by femtosecond direct laser write", Proc. SPIE 11992, Laser 3D Manufacturing IX, 1199208 (4 March 2022); https://doi.org/10.1117/12.2610445
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KEYWORDS
Proteins
Femtosecond phenomena
Multiphoton processes
Microfabrication
Photoresist materials
Printing
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