Photoswitchable stimulated Raman scattering spectroscopy and microscopy

Jingwen Shou; Yasuyuki Ozeki

doi:10.1117/12.2607706

2 March 2022 Photoswitchable stimulated Raman scattering spectroscopy and microscopy

Jingwen Shou, Yasuyuki Ozeki

Proceedings Volume 11957, Biomedical Vibrational Spectroscopy 2022: Advances in Research and Industry; 119570A (2022) https://doi.org/10.1117/12.2607706
Event: SPIE BiOS, 2022, San Francisco, California, United States

Abstract

Photoswitchable fluorescence is a powerful technique to realize super-resolution imaging, highlighting, and optical storage, while its multiplexing capability is limited. Raman scattering is attracting attention because it generates narrowband vibrational signatures, which are potentially useful for highly multiplexed detection of different constituents. Here, we demonstrate photoswitchable SRS spectroscopy and microscopy assisted by photochromic molecules. The narrowband Raman signatures can be switched with full reversibility by applying the irradiation of UV or visible light. The switching speed under low power irradiation is evaluated as fast as within 1 microsecond which is compatible with a conventional point-scan microscope. The demonstration of live-cell imaging suggests the good compatibility to living systems and satisfying sensitivity of this method. We anticipate that photoswitchable SRS imaging will be a powerful foundation for super-multiplex super-resolution imaging.

Conference Presentation

Citation Download Citation

Jingwen Shou and Yasuyuki Ozeki "Photoswitchable stimulated Raman scattering spectroscopy and microscopy", Proc. SPIE 11957, Biomedical Vibrational Spectroscopy 2022: Advances in Research and Industry, 119570A (2 March 2022); https://doi.org/10.1117/12.2607706

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available