Photoacoustic imaging-guided therapy using dual pH/ultrasound-responsive extracellular vesicle-based nanosonosensitizers

Thuy Giang Nguyen Cao; Ji Hee Kang; Wangyu Kim; Junha Lim; Su Jin Kang; Jae Young Yoo; Quan Truong Hoang; Won Jong Kim; Won Jong Rhee; Chulhong Kim; Young Tag Ko; Min Suk Shim

doi:10.1117/12.2609128

3 March 2022 Photoacoustic imaging-guided therapy using dual pH/ultrasound-responsive extracellular vesicle-based nanosonosensitizers

Thuy Giang Nguyen Cao, Ji Hee Kang, Wangyu Kim, Junha Lim, Su Jin Kang, Jae Young Yoo, Quan Truong Hoang, Won Jong Kim, Won Jong Rhee, Chulhong Kim, Young Tag Ko, Min Suk Shim

Author Affiliations +

Proceedings Volume 11938, Visualizing and Quantifying Drug Distribution in Tissue VI; 1193807 (2022) https://doi.org/10.1117/12.2609128
Event: SPIE BiOS, 2022, San Francisco, California, United States

Conference Poster

Abstract

Recently, sonodynamic therapy (SDT) is actively developed, and the reason is that it goes beyond the conventional photodynamic therapy (PDT). SDT demonstrates exceptional ability to treat deep-seated tumor. However, it is difficult to develop sonosensitizers used in SDT. Herein, we demonstrate dual stimuli-responsive theranostic nanosonosensitizers using biocompatible extracellular vesicles (EVs), a class of naturally occurring nanoparticles. With this nanosonosensitizers, we can achieve both high-cancer-targeting and biocompatible properties. Indocyanine green (ICG), used as an sonosensitizer and a photoacoustic (PA) imaging agent, was loaded into EV with paclitaxel (PTX) and sodium bicarbonate (SBC) to achieve pH-responsive PA image-guided chemo-SDT. The SBC trapped in the EV generates CO2 bubbles and causes the EV to burst. That's why SBC-, ICG-, PTX-equipped EVs [ICG/PTX] can efficiently release drugs in response to acidic pH in the endo/lysosomes. The photostability and cellular uptake of ICG was improved by EVs. Further, the ICG-, PTX-loaded EVs and SBCEV( ICG/PTX) showed great anticancer activities against breast cancer cells. The effect of SBC-EV was shown through high resolution PA imaging, which showed that the SBC-EV is accumulated in tumor. SBC-EV (ICG/PTX) with insonification showed that inhibition of tumor growth is possible without toxicity. Thus, this study demonstrates that dual stimuli-responsive SBC-EV(ICG/PTX) holds a great potential for clinical translation of chemo-sonodynamic cancer theranostics.

Conference Presentation

Citation Download Citation

Thuy Giang Nguyen Cao, Ji Hee Kang, Wangyu Kim, Junha Lim, Su Jin Kang, Jae Young Yoo, Quan Truong Hoang, Won Jong Kim, Won Jong Rhee, Chulhong Kim, Young Tag Ko, and Min Suk Shim "Photoacoustic imaging-guided therapy using dual pH/ultrasound-responsive extracellular vesicle-based nanosonosensitizers", Proc. SPIE 11938, Visualizing and Quantifying Drug Distribution in Tissue VI, 1193807 (3 March 2022); https://doi.org/10.1117/12.2609128

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available