Presentation + Paper
3 March 2022 Morpho-molecular characterization of a tattooed skin biopsy using co-localized line-field confocal optical coherence tomography (LC-OCT) and confocal Raman microspectroscopy
Author Affiliations +
Abstract
Line-field confocal optical coherence tomography (LC-OCT) is an optical technique generating three-dimensional (3D) images of the skin at cellular resolution. Confocal Raman microspectroscopy (CRM) is an optical modality that provides a point-wise molecular fingerprint of samples. We have developed a method to co-localize data acquired by separate LC-OCT and CRM systems. LC-OCT allows for recording 3D morphological overview images in which points of interest (POIs) can be localized for molecular analysis using CRM. A biopsy of skin with a red-colored tattoo with unknown ink composition was analyzed using co-localized LC-OCT and CRM. After acquisition of a 3D LC-OCT image, specific POIs were targeted inside the biopsy, based on their morphological features: POIs located in the epidermis where no ink was expected to be found as well as POIs located in bright areas of the dermis, down to 275 μm in depth, likely indicating the presence of tattoo ink. Analysis of the spectra at these specific POIs confirmed the absence of tattoo ink in the epidermis and its presence in the bright areas in the dermis. A stronger molecular signal of ink in the brightest areas of the dermis identified with LC-OCT was also revealed, suggesting a higher amount of ink. The combination and co-localization of LC-OCT and CRM brings a new level of characterization of the skin, enabling molecular analysis of POIs based on their morphological aspect at cellular-resolution within a volume, which could be of great interest in dermatology and dermo-cosmetics.
Conference Presentation
© (2022) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Léna Waszczuk, Jonas Ogien, Jean-Luc Perrot, and Arnaud Dubois "Morpho-molecular characterization of a tattooed skin biopsy using co-localized line-field confocal optical coherence tomography (LC-OCT) and confocal Raman microspectroscopy", Proc. SPIE 11934, Photonics in Dermatology and Plastic Surgery 2022, 1193403 (3 March 2022); https://doi.org/10.1117/12.2607277
Advertisement
Advertisement
RIGHTS & PERMISSIONS
Get copyright permission  Get copyright permission on Copyright Marketplace
KEYWORDS
Raman spectroscopy

Skin

Biopsy

Confocal microscopy

3D image processing

Optical coherence tomography

Imaging spectroscopy

Back to Top