Optical control of cellular signaling pathways using animal opsins

Hisao Tsukamoto

doi:10.1117/12.2615423

27 October 2021 Optical control of cellular signaling pathways using animal opsins

Hisao Tsukamoto

Proceedings Volume 11925, Biomedical Imaging and Sensing Conference 2021; 119250E (2021) https://doi.org/10.1117/12.2615423
Event: SPIE Technologies and Applications of Structured Light, 2021, Online, Japan

Abstract

Currently, in various biological research fields, optical control of cellular responses is widely utilized, leading to establishing a research field named “optogenetics”. Successful optogenetic studies need both state-of-art optical devices illuminating target issues with a high spatiotemporal resolution and optical control tools (typically photosensitive proteins) driving cellular reactions in a light-dependent manner. Most of optogenetic analyses have used channelrhodopsins, lightsensitive ion channels, as control tools. Alternatively, animal opsins, light-sensitive G protein-coupled receptors, can be used as tools to drive a wide variety of G protein-dependent intracellular signaling pathways. Here, I characterize molecular properties of invertebrate opsins by spectroscopic and electrophysiological techniques, and introduce them as “ON-OFF” switch of GPCR signalings. Also, I discuss potentials of the animal opsins as optogenetic tools to expand the research field.

Citation Download Citation

Hisao Tsukamoto "Optical control of cellular signaling pathways using animal opsins", Proc. SPIE 11925, Biomedical Imaging and Sensing Conference 2021, 119250E (27 October 2021); https://doi.org/10.1117/12.2615423

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