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We applied a two laser extraordinary Raman spectroscopy technique in a double nanohole optical tweezers setup to excite and detect the vibrational modes of 20 nm polystyrene beads. We measured the quadrupolar accordion mode frequency to be 47.85 GHz and the spherical breathing mode frequency to be 68.72 GHz which are very close to their predicted values. According to our measurements there is a optimum power to excite the vibrational modes and to get sharp peaks in beat frequency spectrum, simultaneously. Improving the resolution of our present Raman spectroscopy system provides a way to more accurately detect changes in resonance frequency, characterize DNA unzipping, protein-DNA interactions, and classify mutant proteins.
Elham Babaei,Ghazal Haji Salem,Shohei Iwamoto, andReuven Gordon
"Probing optimum applied power for extraordinary acoustic Raman spectroscopy", Proc. SPIE 11798, Optical Trapping and Optical Micromanipulation XVIII, 117981Q (1 August 2021); https://doi.org/10.1117/12.2594964
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