High spatiotemporal resolution live-cell imaging on plasmonic metasurfaces

Kaoru Tamada; Shi Ting Lee; Shihomi Masuda; Thasaneeya Kuboki; Yusuke Arima; Satoru Kidoaki; Koichi Okamoto; Yukiko Aida; Sou Ryuzaki

doi:10.1117/12.2593643

1 August 2021 High spatiotemporal resolution live-cell imaging on plasmonic metasurfaces

Kaoru Tamada, Shi Ting Lee, Shihomi Masuda, Thasaneeya Kuboki, Yusuke Arima, Satoru Kidoaki, Koichi Okamoto, Yukiko Aida, Sou Ryuzaki

Author Affiliations +

Proceedings Volume 11797, Plasmonics: Design, Materials, Fabrication, Characterization, and Applications XIX; 1179709 (2021) https://doi.org/10.1117/12.2593643
Event: SPIE Nanoscience + Engineering, 2021, San Diego, California, United States

Abstract

Recently we have developed LSPR-mediated high resolution live-cell imaging techniques for adhesive cells on plasmonic metasurface composed of self-assembled gold or silver nanoparticles. Compared with other super-resolution microscopy techniques, our LSPR-mediated imaging is extremely simple but quite effective for monitoring the molecular dynamics at a nanointerface. The LSPR of the plasmonic metasurface provides high-contrast interfacial images due to the confined light within a region a few tens of nanometers from the particles and the enhancement of fluorescence. The improved lateral resolution reaches down to the theoretical limit even during cell movement due to the properties of the metasurface with an extremely high refractive index. In the presentation, we will present our latest imaging data concerning molecular dynamics in live cells, which were not visualized by other super-resolution imaging methods with low temporal resolution.

Conference Presentation

Citation Download Citation

Kaoru Tamada, Shi Ting Lee, Shihomi Masuda, Thasaneeya Kuboki, Yusuke Arima, Satoru Kidoaki, Koichi Okamoto, Yukiko Aida, and Sou Ryuzaki "High spatiotemporal resolution live-cell imaging on plasmonic metasurfaces", Proc. SPIE 11797, Plasmonics: Design, Materials, Fabrication, Characterization, and Applications XIX, 1179709 (1 August 2021); https://doi.org/10.1117/12.2593643

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