Three-dimensional and dual-color fluorescence microscopy on a chip

Petra Paiè; Federico Sala; Roberto Memeo; Andrea Bassi; Roberto Osellame; Francesca Bragheri

doi:10.1117/12.2592982

20 June 2021 Three-dimensional and dual-color fluorescence microscopy on a chip

Petra Paiè, Federico Sala, Roberto Memeo, Andrea Bassi, Roberto Osellame, Francesca Bragheri

Proceedings Volume 11786, Optical Methods for Inspection, Characterization, and Imaging of Biomaterials V; 117861Q (2021) https://doi.org/10.1117/12.2592982
Event: SPIE Optical Metrology, 2021, Online Only

Abstract

In this work we present a microscope on chip based on Light Sheet Fluorescence Microscopy, capable to automatically perform 3D and dual-color imaging of specimens diluted in a liquid suspension. A microfluidic channel is used for automatic sample delivery, while integrated optical components such as optical waveguides and lenses are used to illuminate the sample flowing in the channel. The device is fabricated by femtosecond laser micromachining in a glass substrate. Benefiting from the versatility of the fabrication technique we present two prototypes that have been optimized for different samples such as single cells and Drosophila embryos.

Conference Presentation

Citation Download Citation

Petra Paiè, Federico Sala, Roberto Memeo, Andrea Bassi, Roberto Osellame, and Francesca Bragheri "Three-dimensional and dual-color fluorescence microscopy on a chip", Proc. SPIE 11786, Optical Methods for Inspection, Characterization, and Imaging of Biomaterials V, 117861Q (20 June 2021); https://doi.org/10.1117/12.2592982

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