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In order to ensure the detection accuracy of laser-induced fluorescence detection, the micro droplet digital PCR material was required to have weak autofluorescence. The autofluorescence characteristic of micro droplet digital PCR chip was studied in this paper. Firstly, the chip was designed according to the principle of micro droplet digital PCR. Cycloolefin copolymers (COP) and cycloolefin polymers (COP) in organic polymers were selected as the materials of the chip because of their excellent biological characteristics. The laser induced fluorescence device was build. The excitation laser wavelength were FAM (Emission band:522nm, Excitation band:470nm) and HEX (Emission band:553nm, Excitation band:530nm) respectively. The fluorescence image of chip under different exposure time and different band was extracted by COMS camera. And read the fluorescence value through the computer software ImageJ. The glass plate was used as the reference for autofluorescence measurement of chip. From the test results, COC chip showed a good autofluorescence property compared with COP chip, which has lower autofluorescence value under laser excitation. Therefore, for the quantitative detection of trace nucleic acid, the high fluorescent background noise of COP chip will increase the threshold of negative positive discrimination which cloud lead to discrimination error and influence the requirements of detection accuracy. The research results provide a basis for the selection of high-precision micro droplet digital PCR chip materials.
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