Aptasensor based on specific binding induced impedance changes in nanoporous anodized alumina membranes

Nianyu Jiang; Soma Banerjee; Marit Nilsen-Hamilton; Pranav Shrotriya

doi:10.1117/12.2586170

12 April 2021 Aptasensor based on specific binding induced impedance changes in nanoporous anodized alumina membranes

Nianyu Jiang, Soma Banerjee, Marit Nilsen-Hamilton, Pranav Shrotriya

Proceedings Volume 11749, Chemical, Biological, Radiological, Nuclear, and Explosives (CBRNE) Sensing XXII; 117490H (2021) https://doi.org/10.1117/12.2586170
Event: SPIE Defense + Commercial Sensing, 2021, Online Only

Abstract

Monitoring electrochemical impedance changes due to aptamer/ligand binding on nanochannel surface provides an attractive sensing mechanism for chemical and biological species. We report a surface charge modulation based sensing mechanism for the detection of small molecules – amodiaquine and homoserine lactone with their respective specifically binding aptamers. The change in charge distribution due to aptamer/ligand binding modifies ionic transport across the nanochannel, results in transmembrane impedance changes proportional to the amount of target present, providing a quantitative response. A sensor reader based on an analog devices chip ADuCM355 was developed and used to monitor the nanoporous membrane's impedance changes over the desired frequency range. These results show that the combination of a low cost sensor reader and aptamer functionalized membrane will enable the development of a portable and inexpensive sensor system.

Conference Presentation

Citation Download Citation

Nianyu Jiang, Soma Banerjee, Marit Nilsen-Hamilton, and Pranav Shrotriya "Aptasensor based on specific binding induced impedance changes in nanoporous anodized alumina membranes", Proc. SPIE 11749, Chemical, Biological, Radiological, Nuclear, and Explosives (CBRNE) Sensing XXII, 117490H (12 April 2021); https://doi.org/10.1117/12.2586170

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