Digital micro-mirror device based laser-illumination Fourier ptychographic microscopy for high-speed and high-resolution Imaging

Yi Xiao; Shiyuan Wei; Renjie Zhou

doi:10.1117/12.2579176

5 March 2021 Digital micro-mirror device based laser-illumination Fourier ptychographic microscopy for high-speed and high-resolution Imaging

Yi Xiao, Shiyuan Wei, Renjie Zhou

Author Affiliations +

Proceedings Volume 11698, Emerging Digital Micromirror Device Based Systems and Applications XIII; 116980D (2021) https://doi.org/10.1117/12.2579176
Event: SPIE OPTO, 2021, Online Only

Abstract

We propose and demonstrate a digital micro-mirror device (DMD) based laser-illumination Fourier ptychographic microscopy (FPM) method for high-speed and high-resolution label-free imaging applications. Using this method, we can simultaneously retrieve phase and intensity images of samples, such as stained and unstained cancer tissue slices and cells. The system illumination is provided by a 532 nm laser, while two DMDs are utilized for achieving active illumination angle scanning and dynamic filtering. Our current system has achieved real-time imaging at 42 fps with around 1 𝜇𝑚 resolution.

Conference Presentation

Citation Download Citation

Yi Xiao, Shiyuan Wei, and Renjie Zhou "Digital micro-mirror device based laser-illumination Fourier ptychographic microscopy for high-speed and high-resolution Imaging", Proc. SPIE 11698, Emerging Digital Micromirror Device Based Systems and Applications XIII, 116980D (5 March 2021); https://doi.org/10.1117/12.2579176

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