Super-resolution manipulation & imaging of plasmonic CRISPR-Cas9

Guangjie Cui; Zhijia Zhang; Xintao Zhao; Yunbo Liu; Somin Eunice Lee

doi:10.1117/12.2578389

8 March 2021 Super-resolution manipulation & imaging of plasmonic CRISPR-Cas9

Guangjie Cui, Zhijia Zhang, Xintao Zhao, Yunbo Liu, Somin Eunice Lee

Proceedings Volume 11659, Colloidal Nanoparticles for Biomedical Applications XVI; 1165912 (2021) https://doi.org/10.1117/12.2578389
Event: SPIE BiOS, 2021, Online Only

Abstract

CRISPR-Cas9 is a gene editing tool which has promise for the treatment and prevention of many complex diseases. Together with a short guide RNA, CRISPR-Cas9 can recognize and cut the corresponding DNA at a targeted location. Viruses are traditionally used as carriers of CRISPR-Cas9 into target cells. However, viruses may cause immunogenic complications. Alternatively, gold nanoparticles can be utilized as carriers of CRISPR-Cas9. Gold nanoparticles in the near infrared regime exhibit unique optical properties. In this work, we show gold nanoparticles can be stably and efficiently crosslinked to the CRISPR complex.These alternative carriers are toxicity free to and allow effective and efficient introduction of the CRISPR system.

Conference Presentation

Citation Download Citation

Guangjie Cui, Zhijia Zhang, Xintao Zhao, Yunbo Liu, and Somin Eunice Lee "Super-resolution manipulation & imaging of plasmonic CRISPR-Cas9", Proc. SPIE 11659, Colloidal Nanoparticles for Biomedical Applications XVI, 1165912 (8 March 2021); https://doi.org/10.1117/12.2578389

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