High-content, high-throughput imaging of single-cell metabolism by sub-sampled hyperspectral SRS microscopy

Haonan Lin; Yuying Tan; Hyeon Jeong Lee; Ji-Xin Cheng

doi:10.1117/12.2578554

5 March 2021 High-content, high-throughput imaging of single-cell metabolism by sub-sampled hyperspectral SRS microscopy

Haonan Lin, Yuying Tan, Hyeon Jeong Lee, Ji-Xin Cheng

Author Affiliations +

Proceedings Volume 11656, Advanced Chemical Microscopy for Life Science and Translational Medicine 2021; 116560R (2021) https://doi.org/10.1117/12.2578554
Event: SPIE BiOS, 2021, Online Only

Abstract

Hyperspectral stimulated Raman scattering (hSRS) is a label-free microspectroscopic modality that enables live-cell metabolic imaging with chemical specificity. Yet, hSRS in the CH region has low throughput and poor chemical specificity, which limits its application to a broader range of metabolic studies. We propose a high-content, high-throughput hSRS imaging method by a sparsity-driven spectral unmixing and active spectral sub-sampling. We unprecedently generate chemical maps of four major metabolic species (lipid, protein, nucleic acid and carbohydrate) in a Mia PaCa-2 cell using seven spectral frames in the CH region, improving the acquisition speed by over an order of magnitude.

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Haonan Lin, Yuying Tan, Hyeon Jeong Lee, and Ji-Xin Cheng "High-content, high-throughput imaging of single-cell metabolism by sub-sampled hyperspectral SRS microscopy", Proc. SPIE 11656, Advanced Chemical Microscopy for Life Science and Translational Medicine 2021, 116560R (5 March 2021); https://doi.org/10.1117/12.2578554

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KEYWORDS

Microscopy

Mode conditioning cables

Imaging systems

Proteins

Raman scattering

Visualization

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