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5 March 2021 Stimulated Raman voltage imaging for quantitative mapping of membrane potential
Hyeon Jeong Lee, Ji-Xin Cheng
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Proceedings Volume 11656, Advanced Chemical Microscopy for Life Science and Translational Medicine 2021; 1165609 (2021) https://doi.org/10.1117/12.2578015
Event: SPIE BiOS, 2021, Online Only
Abstract
Voltage imaging has become an emerging technique to record membrane potential change in living cells. Yet, compared to electrophysiology, microscopy approaches are still limited to relative membrane voltage changes, lacking important information conveyed by absolute membrane voltage. This talk will cover a spectroscopy approach to tackle this challenge. A spectroscopic signature of membrane potential was identified through stimulated Raman scattering (SRS) imaging, which enabled label-free, sub-cellular voltage imaging of mammalian neurons. We also employed pre-resonance electronic absorption to enhance SRS imaging sensitivity and specificity. microbial rhodopsin voltage sensors, providing a quantitative approach to measure membrane potential. Quantitative voltage imaging by SRS has enabled mapping of absolute voltage and has great potential in neurology and brain sciences.
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Hyeon Jeong Lee and Ji-Xin Cheng "Stimulated Raman voltage imaging for quantitative mapping of membrane potential", Proc. SPIE 11656, Advanced Chemical Microscopy for Life Science and Translational Medicine 2021, 1165609 (5 March 2021); https://doi.org/10.1117/12.2578015
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KEYWORDS
Raman spectroscopy
Neurons
Signal to noise ratio
Visualization
Chromophores
Denoising
Hyperspectral imaging
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