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Tomographic phase microscopy (TPM) is a well-known technique for imaging cells without staining and visualizing their inherent 3-D refractive index profile. We propose a new low-cost TPM approach using sample rotation of single cells. The cells are inserted into a capillary and trapped in a low-density gel. Cell rotation is performed by mechanically rotating the capillary. Projection acquisition is performed using an imaging interferometer. This technique is expected to be useful for label-free cell sorting and analysis.
Matan Dudaie,Itay Barnea, andNatan T. Shaked
"Label-free 3D refractive index interferometric tomography of live cells with full angular coverage", Proc. SPIE 11655, Label-free Biomedical Imaging and Sensing (LBIS) 2021, 116550W (5 March 2021); https://doi.org/10.1117/12.2578229
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Matan Dudaie, Itay Barnea, Natan T. Shaked, "Label-free 3D refractive index interferometric tomography of live cells with full angular coverage," Proc. SPIE 11655, Label-free Biomedical Imaging and Sensing (LBIS) 2021, 116550W (5 March 2021); https://doi.org/10.1117/12.2578229