Label-free multiphoton nonlinear microspectroscopy imaging of microbial cells and organic matter in rhizosphere and soil minerals

Janghyuk Lee; Sonny S. Ly; Ted A. Laurence; Peter K. Weber

doi:10.1117/12.2577311

5 March 2021 Label-free multiphoton nonlinear microspectroscopy imaging of microbial cells and organic matter in rhizosphere and soil minerals

Janghyuk Lee, Sonny S. Ly, Ted A. Laurence, Peter K. Weber

Author Affiliations +

Proceedings Volume 11655, Label-free Biomedical Imaging and Sensing (LBIS) 2021; 116550K (2021) https://doi.org/10.1117/12.2577311
Event: SPIE BiOS, 2021, Online Only

Abstract

We have developed a novel multiphoton nonlinear microscopy with a highly integrated optical imaging system that offers numerous label-free techniques including two-photon excited fluorescence, second-harmonic generation, third-harmonic generation, fluorescence lifetime imaging, and spectral focusing coherent anti-Stokes Raman scattering in one platform. We have applied our system to investigate plant-microbe-mineral interactions in the rhizosphere. The system provides time efficient monitoring of the rhizosphere, offering an array of simultaneous biomolecular information without staining, three-dimensional sub-micron resolution with deeper penetration , and less photodamage. We believe that multiphoton nonlinear optical microscopy will become a valuable imaging tool in the rhizosphere and soil mineral sciences.

Conference Presentation

Citation Download Citation

Janghyuk Lee, Sonny S. Ly, Ted A. Laurence, and Peter K. Weber "Label-free multiphoton nonlinear microspectroscopy imaging of microbial cells and organic matter in rhizosphere and soil minerals", Proc. SPIE 11655, Label-free Biomedical Imaging and Sensing (LBIS) 2021, 116550K (5 March 2021); https://doi.org/10.1117/12.2577311

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available