We propose a multimodal imaging system, LS-GLIM, consisting of the light scanning microscope (LSM 900, Zeiss) and the gradient light interference microscope (GLIM) module [1], with photomultiplier tube (PMT) detection. GLIM upgrades a differential interference microscope with a liquid crystal variable retarder (LCVR), which introduces further controlled phase shifts between the x and y polarized light. The quantitative phase information can be retrieved from the four frames corresponding to each phase shift in the GLIM. We anticipate a broad range of applications that LS-GLIM will enable through phase imaging with computational specificity (PICS), especially in thick, highly-scattering samples.
|