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Microplastics’ occurrence is a major contributor to aquatic pollution and threat to marine ecosystems because these contaminants persist in the environment for hundreds of years. Although there have numerous workflows focusing on identification and characterization of microplastics, standardized methods are lacking. Infrared (IR) Microscopy has been widely used to identify and differentiate microplastics. It has proven to be an excellent technique for quick and accurate analysis; however, lack of standardization in methods and measurement parameters have made incredibly challenging to compare and replicate studies in a systematic manner. In this work, we have laid out systematic experimental methods and parameters for accurate and reproducible microplastics studies using IR Microscopy. We also investigated the lowest measurable size of microplastics using the technique with statistical analysis and multiple experimental approaches to support the findings.
Sudhir Dahal
"Towards standardization of method for microplastics analysis using infrared microscopy", Proc. SPIE 11635, Optical Fibers and Sensors for Medical Diagnostics, Treatment and Environmental Applications XXI, 1163519 (5 March 2021); https://doi.org/10.1117/12.2582570
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Sudhir Dahal, "Towards standardization of method for microplastics analysis using infrared microscopy," Proc. SPIE 11635, Optical Fibers and Sensors for Medical Diagnostics, Treatment and Environmental Applications XXI, 1163519 (5 March 2021); https://doi.org/10.1117/12.2582570