Fluorescence imaging for surgical guidance is a proven modality that allows for visualization of fluorescent markers in numerous biological imaging applications, from cancer margins to tissue oxygenation. As the field continues to develop there is an urgent need for fluorescence-imaging standards that enable system characterization and performance monitoring. Here, we present an update on the proposed indocyanine green (ICG) matching imaging standard. The proposed standard is composed of three different tests: a varying concentration sensitivity test (1 nM-1000 nM), a tissue-equivalent-depth sensitivity test (0.5 mm-6 mm), and a 1951 USAF fluorescence resolution test. The new version of the standard incorporates a fully 3D printed design, which in which includes fluorescent, tissue-equivalent, and optically opaque material. Furthermore we provide photostability and NIST-traceable radiometric measurements.
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