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Fluorescence-guided surgery give the surgeons extra input to improve the outcome of tumor resection procedures. However, the analysis of fluorescence images is qualitative and subjective inputs such as the surgeon’s perception and experience are considered when assessing the tumor margins. Objective indicators are needed to assess accurately the amount of fluorophore within the tissues. We developed a multimodal imaging platform capable of widefield quantitative fluorescence imaging for the use in a clinical environment. By mapping the fluorophore concentration, we offer an objective input for distinguishing healthy from diseased tissue and determining the resections margins in the optimal way: removing cancerous tissue while preserving healthy tissue and vital structures.
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Silvère Ségaud, Labrinus van Manen, Lysanne D. A. N. de Muynck, J. Sven D. Mieog, Alexander L. Vahrmeijer, Sylvain Lecler, Sylvain Gioux, "Widefield quantitative fluorescence imaging applied to tumor margin assessment," Proc. SPIE 11625, Molecular-Guided Surgery: Molecules, Devices, and Applications VII, 116250F (5 March 2021); https://doi.org/10.1117/12.2578576