Construction Of A Novel Functional Bacterial Luciferase By Gene Fusion And Its Use As A Gene Marker In Low Light Video Image Analysis.

A. P. Escher; D. O' Kane; J. Lee; W. H.R. Langridge; A. A. Szalay

doi:10.1117/12.962702

22 December 1989 Construction Of A Novel Functional Bacterial Luciferase By Gene Fusion And Its Use As A Gene Marker In Low Light Video Image Analysis.

A. P. Escher, D. O' Kane, J. Lee, W. H.R. Langridge, A. A. Szalay

Author Affiliations +

Proceedings Volume 1161, New Methods in Microscopy and Low Light Imaging; (1989) https://doi.org/10.1117/12.962702
Event: 33rd Annual Technical Symposium, 1989, San Diego, United States

Abstract

We have engineered a two subunit luciferase enzyme into a single functional polypeptide chain using site specific mutagenesis. We have determined, using low light video imaging, that the activity of this novel enzyme is similar to wild type luciferase when synthesized at low temperatures (15-20°C), but that it is sensitive in vivo to higher temperatures. We have used the gene encoding the monomeric bacterial luciferase as a gene marker in prokaryotic and eukaryotic organisms. Combined with low light video image analysis, it is a practical and powerful tool for quantitatively monitoring gene expression in vivo.

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A. P. Escher, D. O' Kane, J. Lee, W. H.R. Langridge, and A. A. Szalay "Construction Of A Novel Functional Bacterial Luciferase By Gene Fusion And Its Use As A Gene Marker In Low Light Video Image Analysis.", Proc. SPIE 1161, New Methods in Microscopy and Low Light Imaging, (22 December 1989); https://doi.org/10.1117/12.962702

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