High-throughput discrimination of cancerous and noncancerous human cell lines by high-speed spontaneous Raman microscopy

Yasuaki Kumamoto; Kentaro Mochizuki; Kosuke Hashimoto; Yoshinori Harada; Hideo Tanaka; Katsumasa Fujita

doi:10.1117/12.2573223

15 June 2020 High-throughput discrimination of cancerous and noncancerous human cell lines by high-speed spontaneous Raman microscopy

Yasuaki Kumamoto, Kentaro Mochizuki, Kosuke Hashimoto, Yoshinori Harada, Hideo Tanaka, Katsumasa Fujita

Author Affiliations +

Proceedings Volume 11521, Biomedical Imaging and Sensing Conference 2020; 1152112 (2020) https://doi.org/10.1117/12.2573223
Event: SPIE Technologies and Applications of Structured Light, 2020, Yokohama, Japan

Abstract

We used only a narrow range, 1397-1501 cm^-1, for high-throughput analysis of cancerous and noncancerous human cell lines by spontaneous Raman microscopy. With baseline-corrected cellwise spectra in this range, two cell lines were discriminated at accuracy higher than 90%. This narrowband measurement allowed reduction of the signal readout time by 24-folds in comparison to a correspondent wideband measurement detecting 536-3132 cm^-1, enabling cell analysis at 2.5 cells/min. To further improve the throughput, we employed detector binning, which allowed reduction not just of the readout time but also of the signal accumulation time with maintaining signal-to-background ratio and the accuracy. Improvement of the imaging speed by this approach reached at 4-folds, enabling a high-throughout analysis at 10 cells/min.

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Yasuaki Kumamoto, Kentaro Mochizuki, Kosuke Hashimoto, Yoshinori Harada, Hideo Tanaka, and Katsumasa Fujita "High-throughput discrimination of cancerous and noncancerous human cell lines by high-speed spontaneous Raman microscopy", Proc. SPIE 11521, Biomedical Imaging and Sensing Conference 2020, 1152112 (15 June 2020); https://doi.org/10.1117/12.2573223

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KEYWORDS

Raman spectroscopy

Microscopy

Sensors

Signal detection

Confocal microscopy

Image acquisition

Biological research

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