Paper
15 June 2020 Phase-contrast optical microscopy using active image processing
Masaki Hisaka
Author Affiliations +
Abstract
Phase-contrast imaging of biological tissues is one of the most important techniques used to investigate biological structures and functions. A phase-contrast optical microscope using oblique illumination and active image processing that is a figure-8-shaped spatial-frequency filter with sign changes has been developed to visualize phase structure of living tissues. This microscope offers promising features including separate phase and amplitude-contrast imaging, spherical aberration-free imaging, and reduced multi-scattering light. We reproduced and evaluated phase-contrast images of biological samples. We also extended the technique to the phase-contrast scanning microscope using annular illumination.
© (2020) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Masaki Hisaka "Phase-contrast optical microscopy using active image processing", Proc. SPIE 11521, Biomedical Imaging and Sensing Conference 2020, 115210A (15 June 2020); https://doi.org/10.1117/12.2573220
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KEYWORDS
Image processing

Optical microscopes

Optical transfer functions

Visualization

Imaging systems

Microscopes

Optical microscopy

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