New FTIR Techniques For Studying Biological Membranes

Mark S. Braiman; K. Jeffry Wilson

doi:10.1117/12.969519

1 December 1989 New FTIR Techniques For Studying Biological Membranes

Mark S. Braiman, K. Jeffry Wilson

Proceedings Volume 1145, 7th Intl Conf on Fourier Transform Spectroscopy; (1989) https://doi.org/10.1117/12.969519
Event: Seventh International Conference on Fourier and Computerized Infrared Spectroscopy, 1989, Fairfax, VA, United States

Abstract

Two new techniques useful for FTIR studies of biolog-ical membranes are discussed. First, we have developed a sensitive new method of obtaining attenuated total reflection (ATR) spectra of unilamellar lipid bilayers in con-tact with bulk water, by coating the bilayers on infrared-transmitting-optical fibers. Second, we have made several improvements in commercially available software for collecting time-resolved FTIR spectra; our modifications result in a substantial increase in data collection speed and a decrease in baseline artifacts in microsecond time-resolved FTIR spectra of biological samples.

Citation Download Citation

Mark S. Braiman and K. Jeffry Wilson "New FTIR Techniques For Studying Biological Membranes", Proc. SPIE 1145, 7th Intl Conf on Fourier Transform Spectroscopy, (1 December 1989); https://doi.org/10.1117/12.969519

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