Imaging Amphotericin B orientation in Candida spp. through polarization stimulated Raman scattering microscopy (Conference Presentation)

Pu-Ting Dong; Yuewei Zhan; Zeina Dagher; Jie Hui; Kai-Chih Huang; Michael K. Mansour; Ji-Xin Cheng

doi:10.1117/12.2546819

10 March 2020 Imaging Amphotericin B orientation in Candida spp. through polarization stimulated Raman scattering microscopy (Conference Presentation)

Pu-Ting Dong, Yuewei Zhan, Zeina Dagher, Jie Hui, Kai-Chih Huang, Michael K. Mansour, Ji-Xin Cheng

Author Affiliations +

Proceedings Volume 11252, Advanced Chemical Microscopy for Life Science and Translational Medicine; 1125207 (2020) https://doi.org/10.1117/12.2546819
Event: SPIE BiOS, 2020, San Francisco, California, United States

Abstract

Candidemia remains the fourth most common cause of nosocomial bloodstream infections. For more than a half-century, amphotericin B (Amp B) has been the last line of defense in the treatment of life-threatening fungal infections. However, during the past several years, severe infections due to Amp B-resistant Candida spp. isolates have been increasingly reported. Here, through polarization stimulated Raman scattering microscopy, Amp B was found to accumulate largely in the cell membrane of Candida spp. in a highly orientated approach, and the interaction between Amp B and ergosterol was investigated as well. Moreover, we found that the correlation between Amp B and ergosterol in Amp B-susceptible Candida spp. is different from that of Amp B-resistant Candida spp., which provides us important information to understand the working mechanism of Amp B, and to achieve fast determination of the Amp B susceptibility of Candida spp.

Conference Presentation

Citation Download Citation

Pu-Ting Dong, Yuewei Zhan, Zeina Dagher, Jie Hui, Kai-Chih Huang, Michael K. Mansour, and Ji-Xin Cheng "Imaging Amphotericin B orientation in Candida spp. through polarization stimulated Raman scattering microscopy (Conference Presentation)", Proc. SPIE 11252, Advanced Chemical Microscopy for Life Science and Translational Medicine, 1125207 (10 March 2020); https://doi.org/10.1117/12.2546819

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