Free-electron-mediated modifications of biomolecules: from photodamage in nonlinear microscopy to intentional photomodification of cells and tissues (Conference Presentation)

Alfred Vogel; Xiao-Xuan Liang; Sebastian Freidank; Norbert Linz

doi:10.1117/12.2549643

9 March 2020 Free-electron-mediated modifications of biomolecules: from photodamage in nonlinear microscopy to intentional photomodification of cells and tissues (Conference Presentation)

Alfred Vogel, Xiao-Xuan Liang, Sebastian Freidank, Norbert Linz

Author Affiliations +

Proceedings Volume 11244, Multiphoton Microscopy in the Biomedical Sciences XX; 112440I (2020) https://doi.org/10.1117/12.2549643
Event: SPIE BiOS, 2020, San Francisco, California, United States

Abstract

Femtosecond laser-induced plasma generation is used surgically and may also cause photodamage in nonlinear microscopy. Photodamage in multiphoton microscopy already starts at irradiances 1.5 times above the value used for autofluorescence imaging but the cavitation bubble threshold is 20 x higher. We explore the realm of low-density plasma effects between multi-pulse nonlinear imaging and single-pulse surgical regime. We characterize the transition from unchanged tissue (emitting autofluorescence) to slightly changed tissue (hyperfluorescence), drastically changed tissue (plasma luminescence) and disintegrated biomolecules (gas bubble formation). By plotting the threshold values in (irradiance, radiant exposure) space, we identified a “safe” region for nonlinear microscopy.

Conference Presentation

Citation Download Citation

Alfred Vogel, Xiao-Xuan Liang, Sebastian Freidank, and Norbert Linz "Free-electron-mediated modifications of biomolecules: from photodamage in nonlinear microscopy to intentional photomodification of cells and tissues (Conference Presentation)", Proc. SPIE 11244, Multiphoton Microscopy in the Biomedical Sciences XX, 112440I (9 March 2020); https://doi.org/10.1117/12.2549643

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