Oblique plane microscope plate reader for time lapse 3D imaging of live cells in collagen (Conference Presentation)

Nathan Curry; Hugh Sparks; Lucas Dent; Vicky Bousgouni; Vincent Maioli; Ranjeet Kumar; Sunil Kumar; Chris Bakal; Christopher W. Dunsby

doi:10.1117/12.2542691

9 March 2020 Oblique plane microscope plate reader for time lapse 3D imaging of live cells in collagen (Conference Presentation)

Nathan Curry, Hugh Sparks, Lucas Dent, Vicky Bousgouni, Vincent Maioli, Ranjeet Kumar, Sunil Kumar, Chris Bakal, Christopher W. Dunsby

Author Affiliations +

Proceedings Volume 11243, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XVIII; 112430Z (2020) https://doi.org/10.1117/12.2542691
Event: SPIE BiOS, 2020, San Francisco, California, United States

Abstract

Light sheet fluorescence microscopy (LSFM) enables fast 3D imaging of live cells, however the traditional LSFM geometry is not compatible with conventional multiwell plates used in high content microscopy. We have developed an automated LSFM platereader based on an oblique plane microscope (OPM) that is compatible with multiwell plates. The system enables automated studies of cells cultured in a collagen matrix. Cells across 30 different conditions are imaged every 5 minutes for 12 hours. A custom 3D segmentation and tracking pipeline analyses cell morphological dynamics, allowing the study of a range of treatment conditions on the ability of cancer cells to change shape and invade.

Conference Presentation

Citation Download Citation

Nathan Curry, Hugh Sparks, Lucas Dent, Vicky Bousgouni, Vincent Maioli, Ranjeet Kumar, Sunil Kumar, Chris Bakal, and Christopher W. Dunsby "Oblique plane microscope plate reader for time lapse 3D imaging of live cells in collagen (Conference Presentation)", Proc. SPIE 11243, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XVIII, 112430Z (9 March 2020); https://doi.org/10.1117/12.2542691

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