Observations of the bowel wall in the case of acute ischemia: optical coherence tomography, FLIM macro-imaging and histological analysis data

Elena B. Kiseleva; Maxim Ryabkov; Evgenia L. Bederina; Marina V. Shirmanova; Mikhail S. Baleev; Maria M. Lukina; Marina A. Sirotkina; Aleksandra S. Chilipenok; Vladimir V. Beschastnov; Alexander A. Moiseev; Elena V. Zagaynova; Natalia D. Gladkova

doi:10.1117/12.2550667

17 February 2020 Observations of the bowel wall in the case of acute ischemia: optical coherence tomography, FLIM macro-imaging and histological analysis data

Elena B. Kiseleva, Maxim Ryabkov, Evgenia L. Bederina, Marina V. Shirmanova, Mikhail S. Baleev, Maria M. Lukina, Marina A. Sirotkina, Aleksandra S. Chilipenok, Vladimir V. Beschastnov, Alexander A. Moiseev, Elena V. Zagaynova, Natalia D. Gladkova

Author Affiliations +

Proceedings Volume 11232, Multimodal Biomedical Imaging XV; 112320M (2020) https://doi.org/10.1117/12.2550667
Event: SPIE BiOS, 2020, San Francisco, California, United States

Abstract

The objective of the research was a multifaceted study of strangulated small intestine to reveal the optical, morphofunctional and biochemical signs of small bowel ischemia. The study was carried out in vivo using an artificially induced strangulation model of the small intestine (together with its mesentery and blood vessels) in 12 Wistar rats. Over a period of 120 minutes following the bowel ligation, changes in the density of the intramural vasculature and intestinal wall microstructure were detected using multimodal optical coherence tomography (MM OCT). Fluorescence lifetime changes of endogenous fluorophores were also measured using macro-FLIM of the strangulated loop and the adjacent segments of the intestine. At the end of the experiment, a morphometric study of the thickness of the layers and the prevalence of necrosis in the intestinal wall was carried out. A comprehensive analysis of the results of the OCT, FLIM and morphometry of the ischemic wall of the small intestine made it possible to determine the correlating morphofunctional and biochemical manifestations that are specific to this model of mesenteric blood flow disturbance.

Citation Download Citation

Elena B. Kiseleva, Maxim Ryabkov, Evgenia L. Bederina, Marina V. Shirmanova, Mikhail S. Baleev, Maria M. Lukina, Marina A. Sirotkina, Aleksandra S. Chilipenok, Vladimir V. Beschastnov, Alexander A. Moiseev, Elena V. Zagaynova, and Natalia D. Gladkova "Observations of the bowel wall in the case of acute ischemia: optical coherence tomography, FLIM macro-imaging and histological analysis data", Proc. SPIE 11232, Multimodal Biomedical Imaging XV, 112320M (17 February 2020); https://doi.org/10.1117/12.2550667

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
5 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Intestine

Optical coherence tomography

Blood vessels

Ischemia

Fluorescence lifetime imaging

Luminescence

Tissues

Show All Keywords

Keywords/Phrases

Search In:

Publication Years