Femtosecond third-order sum frequency and four-wave mixing imaging

Evan Perillo; M. Lisa Phipps; Jennifer S. Martinez; Anatoly Efimov

doi:10.1117/12.2546261

2 March 2020 Femtosecond third-order sum frequency and four-wave mixing imaging

Evan Perillo, M. Lisa Phipps, Jennifer S. Martinez, Anatoly Efimov

Proceedings Volume 11216, Multiscale Imaging and Spectroscopy; 1121610 (2020) https://doi.org/10.1117/12.2546261
Event: SPIE BiOS, 2020, San Francisco, California, United States

Abstract

Various contrast mechanisms in imaging applications in biological and material sciences are of great importance for multimodal sample visualization. Nonlinear-optical interactions in the sample provide multitude of possibilities to imaging with different contrasts, including sensitivity to chemically-specific vibrational signatures. Cubic order nonlinearity is present in all materials since it does not require broken inversion symmetry. Cubic non- linearity offers several useful interaction modalities, including vibrationally resonant ones, such as third-order sum-frequency generation (TSFG) and four-wave mixing (FWM), which we explore in this work using femtosecond lasers in a laser-scanning all-reflective microscope. We observe strong dependence of image contrast on delay between interacting pulses and the frequency of the mid-IR laser relative to the CH vibrational mode of the sample. Images of oil-water interfaces demonstrate striking visual contrast and impressive signal-to-noise ratio in our system. Pathways to expand TSFG and FWM imaging onto biological samples are explored.

Citation Download Citation

Evan Perillo, M. Lisa Phipps, Jennifer S. Martinez, and Anatoly Efimov "Femtosecond third-order sum frequency and four-wave mixing imaging", Proc. SPIE 11216, Multiscale Imaging and Spectroscopy, 1121610 (2 March 2020); https://doi.org/10.1117/12.2546261

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