Nonlinear optical microscopy of lipogenesis and metabolism in HER2+ breast cancer (Conference Presentation)

Sean Fitzgerald; Giju Thomas; Anita Mahadevan-Jansen

doi:10.1117/12.2546467

9 March 2020 Nonlinear optical microscopy of lipogenesis and metabolism in HER2+ breast cancer (Conference Presentation)

Sean Fitzgerald, Giju Thomas, Anita Mahadevan-Jansen

Author Affiliations +

Proceedings Volume 11216, Multiscale Imaging and Spectroscopy; 112160D (2020) https://doi.org/10.1117/12.2546467
Event: SPIE BiOS, 2020, San Francisco, California, United States

Abstract

Innate differences among breast cancer phenotypes are often studied from the perspective of single protein expressions or by fluorescent imaging using molecular tags. While these techniques can offer useful insights into breast cancer taxonomy, they do not easily translate to clinical care. Nonlinear optical microscopy has revolutionized our ability to study biochemical processes, as it offers a label-free approach to study differences in cancer phenotypes that may provide insight into factors affecting prognosis and treatment strategies. The endogenous chemical specificity offered by nonlinear imaging modalities like Stimulated Raman Scattering (SRS) and Multiphoton Fluorescence (MPF) are attractive alternatives to fluorescent imaging to study intracellular biochemistry. Here we utilize a multimodal imaging platform to characterize lipogenesis in HER2+ cell lines through SRS and metabolic activity by MPF of NADH/FAD to investigate unique signals related to HER2 status.

Conference Presentation

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Sean Fitzgerald, Giju Thomas, and Anita Mahadevan-Jansen "Nonlinear optical microscopy of lipogenesis and metabolism in HER2+ breast cancer (Conference Presentation)", Proc. SPIE 11216, Multiscale Imaging and Spectroscopy, 112160D (9 March 2020); https://doi.org/10.1117/12.2546467

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KEYWORDS

Tumors

Breast cancer

Mammography

Phototherapy

3D modeling

3D image processing

Cancer

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