Estimation of the collagen and elastin condition at lymphedema using multiphoton microscopy

Viktor V. Nikolaev; Ekaterina A. Sandykova; Oksana S. Kurochkina; Denis A. Vrazhnov; Natalia A. Krivova; Yury V. Kistenev; Elena S. Sim

doi:10.1117/12.2540604

18 December 2019 Estimation of the collagen and elastin condition at lymphedema using multiphoton microscopy

Viktor V. Nikolaev, Ekaterina A. Sandykova, Oksana S. Kurochkina, Denis A. Vrazhnov, Natalia A. Krivova, Yury V. Kistenev, Elena S. Sim

Author Affiliations +

Proceedings Volume 11208, 25th International Symposium on Atmospheric and Ocean Optics: Atmospheric Physics; 112080E (2019) https://doi.org/10.1117/12.2540604
Event: XXV International Symposium, Atmospheric and Ocean Optics, Atmospheric Physics, 2019, Novosibirsk, Russian Federation

Abstract

Methods of two-photon microscopy are widely used in the study of biological objects, in particular, skin, due to the possibility to study objects both on the surface and at depth without attracting additional fluorophores due to endogenous autofluorescence. In this paper, the methods of image analysis of the AF signal and SHG signal are applied to assess the condition of the skin during the development of lymphedema. It is shown that for groups of healthy tissue and lymphedematous using SAAID distribution histograms, changes in tissues can be detected.

Citation Download Citation

Viktor V. Nikolaev, Ekaterina A. Sandykova, Oksana S. Kurochkina, Denis A. Vrazhnov, Natalia A. Krivova, Yury V. Kistenev, and Elena S. Sim "Estimation of the collagen and elastin condition at lymphedema using multiphoton microscopy", Proc. SPIE 11208, 25th International Symposium on Atmospheric and Ocean Optics: Atmospheric Physics, 112080E (18 December 2019); https://doi.org/10.1117/12.2540604

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available