Hydrogen peroxide level in tumor cells during cisplatin-induced apoptosis

Anastasiya S. Nerush; Kseniya M. Shсhukina; Irina V. Balalaeva; Anna G. Orlova

doi:10.1117/12.2527195

22 July 2019 Hydrogen peroxide level in tumor cells during cisplatin-induced apoptosis

Anastasiya S. Nerush, Kseniya M. Shсhukina, Irina V. Balalaeva, Anna G. Orlova

Proceedings Volume 11079, Medical Laser Applications and Laser-Tissue Interactions IX; 110791M (2019) https://doi.org/10.1117/12.2527195
Event: European Conferences on Biomedical Optics, 2019, Munich, Germany

Abstract

Using flow cytometry with a genetically encoded fluorescent sensor, vital dye and two types of apoptosis markers: PE Annexin V and TMRE, the participation of hydrogen peroxide (H₂O₂) in cisplatin-induced apoptosis was demonstrated. It was shown that cisplatin causes the increase of H₂O₂ level in early apoptotic cancer cells. Accumulation of H₂O₂ begins before the externalization of phosphatidylserine but after the loss of mitochondrial membrane potential. Scavenging of H₂O₂ prevents the cisplatin-induced apoptosis.

Citation Download Citation

Anastasiya S. Nerush, Kseniya M. Shсhukina, Irina V. Balalaeva, and Anna G. Orlova "Hydrogen peroxide level in tumor cells during cisplatin-induced apoptosis", Proc. SPIE 11079, Medical Laser Applications and Laser-Tissue Interactions IX, 110791M (22 July 2019); https://doi.org/10.1117/12.2527195

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