Tissue fixation and substrate selection in hyperspectral imaging of murine models

Jošt Stergar; Rok Dolenec; Katja Lakota; Martina Perše; Matija Tomšič; Matija Milanic

doi:10.1117/12.2527071

11 July 2019 Tissue fixation and substrate selection in hyperspectral imaging of murine models

Jošt Stergar, Rok Dolenec, Katja Lakota, Martina Perše, Matija Tomšič, Matija Milanic

Proceedings Volume 11074, Diffuse Optical Spectroscopy and Imaging VII; 110741S (2019) https://doi.org/10.1117/12.2527071
Event: European Conferences on Biomedical Optics, 2019, Munich, Germany

Abstract

Animal models present a specific set of challenges for hyperspectral imaging. Sample fixation using formalin changes the optical properties of tissues. Since tissues in murine models can be thin and translucent, substrate selection also plays a key role in properly setting up an experimental protocol.

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Jošt Stergar, Rok Dolenec, Katja Lakota, Martina Perše, Matija Tomšič, and Matija Milanic "Tissue fixation and substrate selection in hyperspectral imaging of murine models", Proc. SPIE 11074, Diffuse Optical Spectroscopy and Imaging VII, 110741S (11 July 2019); https://doi.org/10.1117/12.2527071

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KEYWORDS

Tissues

Hyperspectral imaging

Reflectivity

RGB color model

Scattering

Tissue optics

Chromophores

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