A pyroelectric-based system for sensing low abundant lactose molecules

R. Rega; J. F. Muñoz Martinez; M. Mugnano; E. Oleandro; O. Gennari; P. Orlando; G. Cabassi; V Pelizzola,; P. Ferraro; S. Grilli

doi:10.1117/12.2531770

21 June 2019 A pyroelectric-based system for sensing low abundant lactose molecules

R. Rega, J. F. Muñoz Martinez, M. Mugnano, E. Oleandro, O. Gennari, P. Orlando, G. Cabassi, V Pelizzola,, P. Ferraro, S. Grilli

Author Affiliations +

Proceedings Volume 11060, Optical Methods for Inspection, Characterization, and Imaging of Biomaterials IV; 1106009 (2019) https://doi.org/10.1117/12.2531770
Event: SPIE Optical Metrology, 2019, Munich, Germany

Abstract

A novel method for sensing low abundant lactose in small sample volumes is proposed. It is based on a pyroelectrodynamic jet (p-jet) system able to concentrate the lactose molecules onto a solid amine support for easy and rapid detection through a fluorescence measurement. The p-jet produces droplets with sub-picoliter volumes accumulated onto a microscale area of the solid support in order to reduce the diffusion limits typically occurring in standard well-based assays. A highly reproducible linear response for lactose was obtained between 2 pM/μL and 10 pM/μL. The great advantage of the technique is the ability to concentrate the molecules directly onto the solid support ready for the readout measurement by a standard fluorescence scanner. No time-consuming and expensive sample treatments are needed. The proposed method is rapid, suitable for repeated use providing a built-in quality assurance.

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Citation Download Citation

R. Rega, J. F. Muñoz Martinez, M. Mugnano, E. Oleandro, O. Gennari, P. Orlando, G. Cabassi, V Pelizzola,, P. Ferraro, and S. Grilli "A pyroelectric-based system for sensing low abundant lactose molecules", Proc. SPIE 11060, Optical Methods for Inspection, Characterization, and Imaging of Biomaterials IV, 1106009 (21 June 2019); https://doi.org/10.1117/12.2531770

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