Highly sensitive chemical microscopy by sensing the thermal effect of infrared absorption (Conference Presentation)

Ji-Xin Cheng

doi:10.1117/12.2509884

4 March 2019 Highly sensitive chemical microscopy by sensing the thermal effect of infrared absorption (Conference Presentation)

Ji-Xin Cheng

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Proceedings Volume 10882, Multiphoton Microscopy in the Biomedical Sciences XIX; 1088203 (2019) https://doi.org/10.1117/12.2509884
Event: SPIE BiOS, 2019, San Francisco, California, United States

Abstract

Multiphoton vibrational microscopy is opening a new window to elucidating the rules of life at molecular level. CARS and SRS microscopy has enabled high-speed vibrational imaging of living cells till video rate. However, the ultimate speed and imaging sensitivity are limited by the ultralow cross section of Raman scattering. We report a highly sensitive, high-speed chemical imaging platform based on visible beam sensing of the thermo-refractive effect of infrared absorption. A lab-built resonant circuit is used to extract the photothermal signal at the IR modulation frequency. A virtual lock-in camera is further developed to enable superfast IR-based wide-field chemical imaging at 1250 frames per second. Structural illumination is deployed to further break the diffraction limit of the visible probing beam. With molecular fingerprint information, micro-molar sensitivity and nanoscale spatial resolution, this platform is able to map drug distribution in a pharmaceutical formulation, metabolic activities inside a living cell and further brings IR spectroscopy to in vivo settings.

Conference Presentation

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Ji-Xin Cheng "Highly sensitive chemical microscopy by sensing the thermal effect of infrared absorption (Conference Presentation)", Proc. SPIE 10882, Multiphoton Microscopy in the Biomedical Sciences XIX, 1088203 (4 March 2019); https://doi.org/10.1117/12.2509884

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