The sub-system validation of solid-state detector in BD Accuri C6 plus flow cytometer

Wei P. Chen; Paldin Bet Eivaz; Tim Petersen; Dinesh Gandhi; Kai Liu; Ningyi D. Luo

doi:10.1117/12.2510807

27 February 2019 The sub-system validation of solid-state detector in BD Accuri C6 plus flow cytometer

Wei P. Chen, Paldin Bet Eivaz, Tim Petersen, Dinesh Gandhi, Kai Liu, Ningyi D. Luo

Proceedings Volume 10870, Design and Quality for Biomedical Technologies XII; 108700A (2019) https://doi.org/10.1117/12.2510807
Event: SPIE BiOS, 2019, San Francisco, California, United States

Abstract

The fluorescents detector sub-system is one of most important sub-systems of fluorescence-activated cell sorting (FACS) flow cytometry. The Photomultiplier Tube (PMT) detector sub-system are used in the fluorescent detection of Becton Dickinson (BD) AccuriTM C6 Plus. For using cost effective solid-state detectors (SSD) sub-system, we have validated SSD sub-system as the replacement sub-system of PMT. BD Bioscience and Pavilion Integration Corporation (PIC) use BD Instruments (Accuri C6 Plus) as test tools to test the SSD of PIC vs. PMT. We further ran the Swap test and Repeatability as well as ran test at the operational temperature range of cytometer. We also ran the Linearity test. All results were passed. Based on these results, BD Bioscience teams will run the biological sample testing to cover the test of CFSE- Proliferation Assay, Cell Cycle Analysis, and Apoptosis.

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Citation Download Citation

Wei P. Chen, Paldin Bet Eivaz, Tim Petersen, Dinesh Gandhi, Kai Liu, and Ningyi D. Luo "The sub-system validation of solid-state detector in BD Accuri C6 plus flow cytometer", Proc. SPIE 10870, Design and Quality for Biomedical Technologies XII, 108700A (27 February 2019); https://doi.org/10.1117/12.2510807

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