Extending the performance of linear and nonlinear spatial frequency modulated imaging platforms  (Conference Presentation)

Jeffrey A. Squier; Nathan Worts; Alyssa Allende Motz; Jeff Field; Keith Wernsing; Patrick Stockton; Randy Bartels

doi:10.1117/12.2321327

17 September 2018 Extending the performance of linear and nonlinear spatial frequency modulated imaging platforms (Conference Presentation)

Jeffrey A. Squier, Nathan Worts, Alyssa Allende Motz, Jeff Field, Keith Wernsing, Patrick Stockton, Randy Bartels

Author Affiliations +

Proceedings Volume 10753, Ultrafast Nonlinear Imaging and Spectroscopy VI; 107530L (2018) https://doi.org/10.1117/12.2321327
Event: SPIE Optical Engineering + Applications, 2018, San Diego, California, United States

Abstract

Through spatial frequency modulated imaging (SPIFI), multimodal, multiphoton microscopy (MPM) benefits from an extended excitation source without compromising the key performance characteristics afforded by point scanning MPM platforms. For example, the introduction of an in-house custom machined mask, which imparts a spatially distinct, temporal amplitude modulation to the extended excitation source, allows one and two-dimensional images to be captured with single element detection. This enables extended source imaging methods to retain a key feature of the point scanning systems; namely, the ability to image within scattering media, at depth. Further, the range of contrast mechanisms for the extended source techniques presented here are not limited and readily extend to both linear and nonlinear imaging modalities. The SPIFI method developed here enables facile detection of such images with the added benefit of enhanced resolution. Notably, the resolution improvement holds across contrast mechanisms, and is independent of whether the contrast is generated through linear or nonlinear processes. Significantly, phase also comes into play as we present new SPIFI geometries that illustrate the role of phase in strategically controlling the source geometry and/or generating image contrast.

Conference Presentation

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Jeffrey A. Squier, Nathan Worts, Alyssa Allende Motz, Jeff Field, Keith Wernsing, Patrick Stockton, and Randy Bartels "Extending the performance of linear and nonlinear spatial frequency modulated imaging platforms (Conference Presentation)", Proc. SPIE 10753, Ultrafast Nonlinear Imaging and Spectroscopy VI, 107530L (17 September 2018); https://doi.org/10.1117/12.2321327

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KEYWORDS

Modulation

Spatial frequencies

Imaging systems

Amplitude modulation

Image resolution

Multimodal imaging

Multiphoton microscopy

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