Characterizing the hydration state of L-threonine in solution using terahertz time-domain attenuated total reflection spectroscopy

Huachuan Huang; Qiao Liu; Liguo Zhu; Zeren Li

doi:10.1117/12.2295512

12 January 2018 Characterizing the hydration state of L-threonine in solution using terahertz time-domain attenuated total reflection spectroscopy

Huachuan Huang, Qiao Liu, Liguo Zhu, Zeren Li

Author Affiliations +

Proceedings Volume 10623, 2017 International Conference on Optical Instruments and Technology: IRMMW-THz Technologies and Applications; 106230N (2018) https://doi.org/10.1117/12.2295512
Event: International Conference on Optical Instruments and Technology 2017, 2017, Beijing, China

Abstract

The hydration of biomolecules is closely related to the dynamic process of their functional expression, therefore, characterizing hydration phenomena is a subject of keen interest. However, direct measurements on the global hydration state of biomolecules couldn’t have been acquired using traditional techniques such as thermodynamics, ultrasound, microwave spectroscopy or viscosity, etc. In order to realize global hydration characterization of amino acid such as L-threonine, terahertz time-domain attenuated total reflectance spectroscopy (THz-TDS-ATR) was adopted in this paper. By measuring the complex permittivity of L-threonine solutions with various concentrations in the THz region, the hydration state and its concentration dependence were obtained, indicating that the number of hydrous water decreased with the increase of concentration. The hydration number was evaluated to be 17.8 when the molar concentration of L-threonine was 0.34 mol/L, and dropped to 13.2 when the molar concentration increased to 0.84 mol/L, when global hydration was taken into account. According to the proposed direct measurements, it is believed that the THz-TDS-ATR technique is a powerful tool for studying the picosecond molecular dynamics of amino acid solutions.

Citation Download Citation

Huachuan Huang, Qiao Liu, Liguo Zhu, and Zeren Li "Characterizing the hydration state of L-threonine in solution using terahertz time-domain attenuated total reflection spectroscopy", Proc. SPIE 10623, 2017 International Conference on Optical Instruments and Technology: IRMMW-THz Technologies and Applications, 106230N (12 January 2018); https://doi.org/10.1117/12.2295512

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available