FT-IR Spectroscopy: The Detection Of Pressure-Induced Changes In The Secondary Structure Of Proteins

Patrick T. T. Wong; Henry H. Mantsch

doi:10.1117/12.951647

8 May 1989 FT-IR Spectroscopy: The Detection Of Pressure-Induced Changes In The Secondary Structure Of Proteins

Patrick T. T. Wong, Henry H. Mantsch

Proceedings Volume 1057, Biomolecular Spectroscopy; (1989) https://doi.org/10.1117/12.951647
Event: OE/LASE '89, 1989, Los Angeles, CA, United States

Abstract

The study of pressure-induced protein:protein interactions, and in particular the pressure-induced denaturation and dissociation of oligomeric proteins, has gained considerable interest in recent years. It is now well recognized that the protein transformation known under the general name of denaturation is a physical process which affects the three dimensional protein structure,leading to chain unfolding and/or refolding. Denaturation can be induced by changes in temperature or pressure, and by the addition of denaturing agents. The rationale for using pressure to study protein denaturation is that pressure variations at constant temperature only affect the volume of the protein, unlike the more commonly used temperature denaturation, which affects both the volume and the kinetic properties of the protein.

Citation Download Citation

Patrick T. T. Wong and Henry H. Mantsch "FT-IR Spectroscopy: The Detection Of Pressure-Induced Changes In The Secondary Structure Of Proteins", Proc. SPIE 1057, Biomolecular Spectroscopy, (8 May 1989); https://doi.org/10.1117/12.951647

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available