Recent Developments And Applications Of Multidimensional Fluorescence Spectroscopy

J. B. Zung; R. L. Woodlee; M.-R. S. Fuh; I. M. Warner

doi:10.1117/12.951544

17 May 1989 Recent Developments And Applications Of Multidimensional Fluorescence Spectroscopy

J. B. Zung, R. L. Woodlee, M.-R. S. Fuh, I. M. Warner

Proceedings Volume 1054, Fluorescence Detection III; (1989) https://doi.org/10.1117/12.951544
Event: OE/LASE '89, 1989, Los Angeles, CA, United States

Abstract

Fluorescence spectroscopy is an inherently selective technique; however, single wavelength measurements can be limited for the analysis of complicated multicomponent samples or even a simple mixture with overlapping emission spectra. To overcome this limitation, multidimensional fluorescence measurements can be used to obtain additional information about the sample. The multidimensional fluorescence measurement involves the use of multiple parameters to increase the specificity of the measurement. In this laboratory, we are especially interested in multiparametric measurements such as the Excitation-Emission Spectrum. In this paper a fiber optic based multidimensional fluorometer is examined along with a portable multichannel fluorcineter in terms of sensitivity. In addition, specific applications incorporating the excitation-emission matrix are presented.

Citation Download Citation

J. B. Zung, R. L. Woodlee, M.-R. S. Fuh, and I. M. Warner "Recent Developments And Applications Of Multidimensional Fluorescence Spectroscopy", Proc. SPIE 1054, Fluorescence Detection III, (17 May 1989); https://doi.org/10.1117/12.951544

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